@PHDTHESIS{ 2021:1340377603,
 	title = {Adenosine Dependent Anti-Inflammatory Effects of Arrabidaea Chica VERLOT},
 	year = {2021},
 	url = "https://tede.ufam.edu.br/handle/tede/8399",
 	abstract = "Arrabidaea Chica (Bigonnnace) is commonly used in The Amazon by folks due to its anti-inflammatory properties, properties that have been confirmed in different experimental
 models of disease, but the molecular processes that govern this effect were unclear. The
 immune system mediates inflammation through a variety of mechanisms, including the
 production of pro-inflammatory and anti-inflammatory cytokines, which are directed by
 different signaling pathways, among them the adenosinergic pathway. The study of new antiinflammatory molecules provides a long-term solution to the global problem that represents
 inflammation when uncontrolled and the Amazon is rich in natural products that need to be
 studied. The objective of this study was to elucidate whether or not the anti-inflammatory
 effect of Arrabidaea Chica VERLOT in bone marrow-derived macrophages (BMDM) is
 mediated by adenosine. The production of pro-inflammatory (IL-1? and TNF?) and anti-inflammatory (IL-10) cytokines, was assessed by ELISA in the supernatant of BMDM preincubated with Arrabidaea ChicaVERLOT aqueous extract and stimulated with LPS.
 Arrabidaea Chica demonstrated to decrease the production of IL-1? and increase the
 production of IL-10. The increased production of IL-10 was associated with elevated
 production of adenosine and this effect was not observed when the different steps of the
 adenosinergic pathway were pharmacologically inhibited or genetically suppressed. The
 participation of the metabolism of the cells was also confirmed as the direct source of ATP
 for the generation of adenosine. We conclude that Arrabidaea Chica VERLOT mediates its
 anti-inflammatory effect via the adenosinergic pathway, increasing the metabolic rate of the
 cells to generate the substrate for this pathway.",
 	publisher = {Universidade Federal do Amazonas},
 	scholl = {Programa de P?s-gradua??o em Imunologia B?sica e Aplicada},
 	note = {Instituto de Ci?ncias Biol?gicas}
}